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Effect of IL-10 and IFN-g on SARS-CoV-2 replication and SARS-CoV-2-induced cytokines, ISGs/chemokines, and <t>IDO1</t> in Calu-3 cells Calu-3 cells were pretreated with IL-10 (A) or IFN-g (B) for 1 h and infected with SARS-CoV-2 (delta variant) with 0.01 MOI for 48 h. RNA was isolated from cell lysate and culture supernatant and analyzed for viral RNA copies using E gene-specific primers and probes by RT-qPCR (A and B). Total cellular RNA was reverse transcribed to cDNA, and gene expression of host genes was analyzed by RT-qPCR (C and D). Data represent the mean ± SEM. ∗ p < 0.05, 0.01 MOI vs. 0.01 MOI + IFN-γ. ns, non-significant.
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Effect of IL-10 and IFN-g on SARS-CoV-2 replication and SARS-CoV-2-induced cytokines, ISGs/chemokines, and <t>IDO1</t> in Calu-3 cells Calu-3 cells were pretreated with IL-10 (A) or IFN-g (B) for 1 h and infected with SARS-CoV-2 (delta variant) with 0.01 MOI for 48 h. RNA was isolated from cell lysate and culture supernatant and analyzed for viral RNA copies using E gene-specific primers and probes by RT-qPCR (A and B). Total cellular RNA was reverse transcribed to cDNA, and gene expression of host genes was analyzed by RT-qPCR (C and D). Data represent the mean ± SEM. ∗ p < 0.05, 0.01 MOI vs. 0.01 MOI + IFN-γ. ns, non-significant.
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Effect of IL-10 and IFN-g on SARS-CoV-2 replication and SARS-CoV-2-induced cytokines, ISGs/chemokines, and IDO1 in Calu-3 cells Calu-3 cells were pretreated with IL-10 (A) or IFN-g (B) for 1 h and infected with SARS-CoV-2 (delta variant) with 0.01 MOI for 48 h. RNA was isolated from cell lysate and culture supernatant and analyzed for viral RNA copies using E gene-specific primers and probes by RT-qPCR (A and B). Total cellular RNA was reverse transcribed to cDNA, and gene expression of host genes was analyzed by RT-qPCR (C and D). Data represent the mean ± SEM. ∗ p < 0.05, 0.01 MOI vs. 0.01 MOI + IFN-γ. ns, non-significant.

Journal: iScience

Article Title: Differential immunometabolic responses to Delta and Omicron SARS-CoV-2 variants in golden syrian hamsters

doi: 10.1016/j.isci.2024.110501

Figure Lengend Snippet: Effect of IL-10 and IFN-g on SARS-CoV-2 replication and SARS-CoV-2-induced cytokines, ISGs/chemokines, and IDO1 in Calu-3 cells Calu-3 cells were pretreated with IL-10 (A) or IFN-g (B) for 1 h and infected with SARS-CoV-2 (delta variant) with 0.01 MOI for 48 h. RNA was isolated from cell lysate and culture supernatant and analyzed for viral RNA copies using E gene-specific primers and probes by RT-qPCR (A and B). Total cellular RNA was reverse transcribed to cDNA, and gene expression of host genes was analyzed by RT-qPCR (C and D). Data represent the mean ± SEM. ∗ p < 0.05, 0.01 MOI vs. 0.01 MOI + IFN-γ. ns, non-significant.

Article Snippet: IDO1 inhibitor (Indoximod) , MedChemExpress , HY-16724.

Techniques: Infection, Variant Assay, Isolation, Quantitative RT-PCR, Reverse Transcription, Expressing

Journal: iScience

Article Title: Differential immunometabolic responses to Delta and Omicron SARS-CoV-2 variants in golden syrian hamsters

doi: 10.1016/j.isci.2024.110501

Figure Lengend Snippet:

Article Snippet: IDO1 inhibitor (Indoximod) , MedChemExpress , HY-16724.

Techniques: Virus, Variant Assay, Recombinant, Avidin-Biotin Assay, Plasmid Preparation, Software

Ongoing clinical trials of immunotherapy for brain tumors.

Journal: Current Oncology

Article Title: The Role of Immunotherapy in the Treatment of Rare Central Nervous System Tumors

doi: 10.3390/curroncol30060401

Figure Lengend Snippet: Ongoing clinical trials of immunotherapy for brain tumors.

Article Snippet: , NCT02502708 , NewLink Genetics Corporation (Ames, IA, US) , Study of the IDO Pathway Inhibitor, Indoximod, and Temozolomide for Pediatric Patients with Progressive Primary Malignant Brain Tumors , Completed, no results posted , US.

Techniques: Clinical Proteomics, Expressing, Modification, Virus, Genetically Modified, Transplantation Assay

A list of the different GBM therapies, associated drug names, companies in charge of their development or commercialization, as well as drug proposed mode of action.

Journal: Frontiers in Pharmacology

Article Title: Glioblastoma Treatments: An Account of Recent Industrial Developments

doi: 10.3389/fphar.2018.00879

Figure Lengend Snippet: A list of the different GBM therapies, associated drug names, companies in charge of their development or commercialization, as well as drug proposed mode of action.

Article Snippet: Indoximod , IDO inhibitor , Newlinkgenetics , Inhibits IDO (indoleamine 2,3-dioxygenase) that inactivates NK (natural killer) cells and generates Tregs (regulatory T cells)..

Techniques: Activity Assay, Binding Assay, Modification, Activation Assay, Injection, Amplification, Generated, Blocking Assay, Virus, Purification, Membrane, Transformation Assay, Saline